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This hyperfused phenotype suggests failure of fission-mediated quality surveillance, allowing genetically compromised mitochondria to persist and thereby impairing oocyte quality, fertilization capacity, and early embryonic development (Khashchenko et al., 2024)
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We used lentivirus to construct the stable cell lines overexpressing MANF, ensuring higher transfection efficiency and repeatability, whereas the previous study adopted transient transfection with FLAG-MANF plasmid, [37]
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