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Similarly, a decreased glycolytic reserve has been found in NK cells from ME/CFS patients (389) and metabolomic analyses have revealed compromised ATP production via the tricarboxylic acid (TCA) cycle (389)
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After derivatization with 10 μ l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 μ l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min
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